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13(s)-hode-d4 standard  (Cayman Chemical)


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    Structured Review

    Cayman Chemical 13(s)-hode-d4 standard
    13(s) Hode D4 Standard, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/13-hode/13+hode+d4/pmc12272432-37-48-73
    Average 90 stars, based on 1 article reviews
    13(s)-hode-d4 standard - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE.
    Article Snippet: Different fatty acids have distinct effects on the survival of breast cancer cells, which could be mediated by fatty acid binding proteins (FABPs), a family of lipid chaperones.. Due to the diverse structures of the members of FABP family, each FABP demonstrates distinct binding affinities to different fatty acids.. Of note, FABP7 is predominantly expressed in triple negative breast cancer (TNBC), the most aggressive subtype of breast cancer.

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Article Title: Characterization of oxylipins in Antarctic krill oil (Euphausia superba) during storage based on RPLC-MS/MS analysis.
    Article Snippet: Antarctic krill oil (AKO) is rich in polyunsaturated fatty acids (PUFAs), but is prone to oxidative degradation, resulting in the formation of oxylipins, which compromise AKO quality.. Herein, we used reversed-phase-high performance liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) to perform qualitative and semi-quantitative analyses of oxylipins in AKO during storage.. A total of 27 oxylipins were identified.

    Article Title: Oxidized linoleic acid metabolites regulate neuronal morphogenesis in vitro
    Article Snippet: 13-HODE (Cayman Chemical, Ann Arbor, MI, USA; Cat #38600); 9-HODE (Cayman Chemical; Cat #38400); 9,10-DiHOME (Cayman Chemical; Cat #53400); 12(13)-EpOME (Cayman Chemical; Cat #52450); 9,10,13-TriHOME (Cayman Chemical; Cat # 26768); 9-OxoODE (Cayman Chemical; Cat #38420); and 12,13-DiHOME (Cayman Chemical; Cat #10009832) were dissolved as 1 mM stocks in absolute ethanol.

    Article Title: Holding glycolysis in check though Alox15 activity is required for macrophage M2 commitment and function in tissue repair and anti-helminth immunity
    Article Snippet: BEC 1 uM (Sigma), Rosiglitazone 0.1, 0.5, 1uM (PPAR-γ agonist, Sigma), GW501516 0.1, 1, 5uM (PPAR-δ agonist, Sigma), n-3 Docosapetaenoic acid sodium salt DPA 100uM (Cayman Chemical) 15-HETE (Cayman Chemical), 13-HODE (Cayman Chemical), 9-HODE (Cayman Chemical) were reconstituted in DMSO (Sigma).

    Article Title: Influence of Lipoxygenase Inhibition on Glioblastoma Cell Biology
    Article Snippet: After 24 h, cells were treated with 0.1 μM, 0.5 μM, and 1 μM of the following (S)-form oxylipins: 13-HODE, 9-HODE, 15-HETE, 5-HETE, 12-HETE, 8-HETE, 9-HETE, 20-HETE (all Cayman Chemical, USA) and 100% ethanol (control).

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: Treatments LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Incubation:

    Article Title: Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE.
    Article Snippet: Different fatty acids have distinct effects on the survival of breast cancer cells, which could be mediated by fatty acid binding proteins (FABPs), a family of lipid chaperones.. Due to the diverse structures of the members of FABP family, each FABP demonstrates distinct binding affinities to different fatty acids.. Of note, FABP7 is predominantly expressed in triple negative breast cancer (TNBC), the most aggressive subtype of breast cancer.

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Article Title: Characterization of oxylipins in Antarctic krill oil (Euphausia superba) during storage based on RPLC-MS/MS analysis.
    Article Snippet: Antarctic krill oil (AKO) is rich in polyunsaturated fatty acids (PUFAs), but is prone to oxidative degradation, resulting in the formation of oxylipins, which compromise AKO quality.. Herein, we used reversed-phase-high performance liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) to perform qualitative and semi-quantitative analyses of oxylipins in AKO during storage.. A total of 27 oxylipins were identified.

    Article Title: Oxidized linoleic acid metabolites regulate neuronal morphogenesis in vitro
    Article Snippet: 13-HODE (Cayman Chemical, Ann Arbor, MI, USA; Cat #38600); 9-HODE (Cayman Chemical; Cat #38400); 9,10-DiHOME (Cayman Chemical; Cat #53400); 12(13)-EpOME (Cayman Chemical; Cat #52450); 9,10,13-TriHOME (Cayman Chemical; Cat # 26768); 9-OxoODE (Cayman Chemical; Cat #38420); and 12,13-DiHOME (Cayman Chemical; Cat #10009832) were dissolved as 1 mM stocks in absolute ethanol.

    Article Title: Holding glycolysis in check though Alox15 activity is required for macrophage M2 commitment and function in tissue repair and anti-helminth immunity
    Article Snippet: BEC 1 uM (Sigma), Rosiglitazone 0.1, 0.5, 1uM (PPAR-γ agonist, Sigma), GW501516 0.1, 1, 5uM (PPAR-δ agonist, Sigma), n-3 Docosapetaenoic acid sodium salt DPA 100uM (Cayman Chemical) 15-HETE (Cayman Chemical), 13-HODE (Cayman Chemical), 9-HODE (Cayman Chemical) were reconstituted in DMSO (Sigma).

    Article Title: Influence of Lipoxygenase Inhibition on Glioblastoma Cell Biology
    Article Snippet: After 24 h, cells were treated with 0.1 μM, 0.5 μM, and 1 μM of the following (S)-form oxylipins: 13-HODE, 9-HODE, 15-HETE, 5-HETE, 12-HETE, 8-HETE, 9-HETE, 20-HETE (all Cayman Chemical, USA) and 100% ethanol (control).

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: Treatments LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Concentration Assay:

    Article Title: Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE.
    Article Snippet: Different fatty acids have distinct effects on the survival of breast cancer cells, which could be mediated by fatty acid binding proteins (FABPs), a family of lipid chaperones.. Due to the diverse structures of the members of FABP family, each FABP demonstrates distinct binding affinities to different fatty acids.. Of note, FABP7 is predominantly expressed in triple negative breast cancer (TNBC), the most aggressive subtype of breast cancer.

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Article Title: Characterization of oxylipins in Antarctic krill oil (Euphausia superba) during storage based on RPLC-MS/MS analysis.
    Article Snippet: Antarctic krill oil (AKO) is rich in polyunsaturated fatty acids (PUFAs), but is prone to oxidative degradation, resulting in the formation of oxylipins, which compromise AKO quality.. Herein, we used reversed-phase-high performance liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) to perform qualitative and semi-quantitative analyses of oxylipins in AKO during storage.. A total of 27 oxylipins were identified.

    Article Title: Oxidized linoleic acid metabolites regulate neuronal morphogenesis in vitro
    Article Snippet: 13-HODE (Cayman Chemical, Ann Arbor, MI, USA; Cat #38600); 9-HODE (Cayman Chemical; Cat #38400); 9,10-DiHOME (Cayman Chemical; Cat #53400); 12(13)-EpOME (Cayman Chemical; Cat #52450); 9,10,13-TriHOME (Cayman Chemical; Cat # 26768); 9-OxoODE (Cayman Chemical; Cat #38420); and 12,13-DiHOME (Cayman Chemical; Cat #10009832) were dissolved as 1 mM stocks in absolute ethanol.

    Article Title: Holding glycolysis in check though Alox15 activity is required for macrophage M2 commitment and function in tissue repair and anti-helminth immunity
    Article Snippet: BEC 1 uM (Sigma), Rosiglitazone 0.1, 0.5, 1uM (PPAR-γ agonist, Sigma), GW501516 0.1, 1, 5uM (PPAR-δ agonist, Sigma), n-3 Docosapetaenoic acid sodium salt DPA 100uM (Cayman Chemical) 15-HETE (Cayman Chemical), 13-HODE (Cayman Chemical), 9-HODE (Cayman Chemical) were reconstituted in DMSO (Sigma).

    Article Title: Influence of Lipoxygenase Inhibition on Glioblastoma Cell Biology
    Article Snippet: After 24 h, cells were treated with 0.1 μM, 0.5 μM, and 1 μM of the following (S)-form oxylipins: 13-HODE, 9-HODE, 15-HETE, 5-HETE, 12-HETE, 8-HETE, 9-HETE, 20-HETE (all Cayman Chemical, USA) and 100% ethanol (control).

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: Treatments LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Positive Control:

    Article Title: Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE.
    Article Snippet: Different fatty acids have distinct effects on the survival of breast cancer cells, which could be mediated by fatty acid binding proteins (FABPs), a family of lipid chaperones.. Due to the diverse structures of the members of FABP family, each FABP demonstrates distinct binding affinities to different fatty acids.. Of note, FABP7 is predominantly expressed in triple negative breast cancer (TNBC), the most aggressive subtype of breast cancer.

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Article Title: Characterization of oxylipins in Antarctic krill oil (Euphausia superba) during storage based on RPLC-MS/MS analysis.
    Article Snippet: Antarctic krill oil (AKO) is rich in polyunsaturated fatty acids (PUFAs), but is prone to oxidative degradation, resulting in the formation of oxylipins, which compromise AKO quality.. Herein, we used reversed-phase-high performance liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) to perform qualitative and semi-quantitative analyses of oxylipins in AKO during storage.. A total of 27 oxylipins were identified.

    Article Title: Oxidized linoleic acid metabolites regulate neuronal morphogenesis in vitro
    Article Snippet: 13-HODE (Cayman Chemical, Ann Arbor, MI, USA; Cat #38600); 9-HODE (Cayman Chemical; Cat #38400); 9,10-DiHOME (Cayman Chemical; Cat #53400); 12(13)-EpOME (Cayman Chemical; Cat #52450); 9,10,13-TriHOME (Cayman Chemical; Cat # 26768); 9-OxoODE (Cayman Chemical; Cat #38420); and 12,13-DiHOME (Cayman Chemical; Cat #10009832) were dissolved as 1 mM stocks in absolute ethanol.

    Article Title: Holding glycolysis in check though Alox15 activity is required for macrophage M2 commitment and function in tissue repair and anti-helminth immunity
    Article Snippet: BEC 1 uM (Sigma), Rosiglitazone 0.1, 0.5, 1uM (PPAR-γ agonist, Sigma), GW501516 0.1, 1, 5uM (PPAR-δ agonist, Sigma), n-3 Docosapetaenoic acid sodium salt DPA 100uM (Cayman Chemical) 15-HETE (Cayman Chemical), 13-HODE (Cayman Chemical), 9-HODE (Cayman Chemical) were reconstituted in DMSO (Sigma).

    Article Title: Influence of Lipoxygenase Inhibition on Glioblastoma Cell Biology
    Article Snippet: After 24 h, cells were treated with 0.1 μM, 0.5 μM, and 1 μM of the following (S)-form oxylipins: 13-HODE, 9-HODE, 15-HETE, 5-HETE, 12-HETE, 8-HETE, 9-HETE, 20-HETE (all Cayman Chemical, USA) and 100% ethanol (control).

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: Treatments LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Control:

    Article Title: Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE.
    Article Snippet: Different fatty acids have distinct effects on the survival of breast cancer cells, which could be mediated by fatty acid binding proteins (FABPs), a family of lipid chaperones.. Due to the diverse structures of the members of FABP family, each FABP demonstrates distinct binding affinities to different fatty acids.. Of note, FABP7 is predominantly expressed in triple negative breast cancer (TNBC), the most aggressive subtype of breast cancer.

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Article Title: Characterization of oxylipins in Antarctic krill oil (Euphausia superba) during storage based on RPLC-MS/MS analysis.
    Article Snippet: Antarctic krill oil (AKO) is rich in polyunsaturated fatty acids (PUFAs), but is prone to oxidative degradation, resulting in the formation of oxylipins, which compromise AKO quality.. Herein, we used reversed-phase-high performance liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) to perform qualitative and semi-quantitative analyses of oxylipins in AKO during storage.. A total of 27 oxylipins were identified.

    Article Title: Oxidized linoleic acid metabolites regulate neuronal morphogenesis in vitro
    Article Snippet: 13-HODE (Cayman Chemical, Ann Arbor, MI, USA; Cat #38600); 9-HODE (Cayman Chemical; Cat #38400); 9,10-DiHOME (Cayman Chemical; Cat #53400); 12(13)-EpOME (Cayman Chemical; Cat #52450); 9,10,13-TriHOME (Cayman Chemical; Cat # 26768); 9-OxoODE (Cayman Chemical; Cat #38420); and 12,13-DiHOME (Cayman Chemical; Cat #10009832) were dissolved as 1 mM stocks in absolute ethanol.

    Article Title: Holding glycolysis in check though Alox15 activity is required for macrophage M2 commitment and function in tissue repair and anti-helminth immunity
    Article Snippet: BEC 1 uM (Sigma), Rosiglitazone 0.1, 0.5, 1uM (PPAR-γ agonist, Sigma), GW501516 0.1, 1, 5uM (PPAR-δ agonist, Sigma), n-3 Docosapetaenoic acid sodium salt DPA 100uM (Cayman Chemical) 15-HETE (Cayman Chemical), 13-HODE (Cayman Chemical), 9-HODE (Cayman Chemical) were reconstituted in DMSO (Sigma).

    Article Title: Influence of Lipoxygenase Inhibition on Glioblastoma Cell Biology
    Article Snippet: After 24 h, cells were treated with 0.1 μM, 0.5 μM, and 1 μM of the following (S)-form oxylipins: 13-HODE, 9-HODE, 15-HETE, 5-HETE, 12-HETE, 8-HETE, 9-HETE, 20-HETE (all Cayman Chemical, USA) and 100% ethanol (control).

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: Treatments LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Produced:

    Article Title: Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE.
    Article Snippet: Different fatty acids have distinct effects on the survival of breast cancer cells, which could be mediated by fatty acid binding proteins (FABPs), a family of lipid chaperones.. Due to the diverse structures of the members of FABP family, each FABP demonstrates distinct binding affinities to different fatty acids.. Of note, FABP7 is predominantly expressed in triple negative breast cancer (TNBC), the most aggressive subtype of breast cancer.

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Article Title: Characterization of oxylipins in Antarctic krill oil (Euphausia superba) during storage based on RPLC-MS/MS analysis.
    Article Snippet: Antarctic krill oil (AKO) is rich in polyunsaturated fatty acids (PUFAs), but is prone to oxidative degradation, resulting in the formation of oxylipins, which compromise AKO quality.. Herein, we used reversed-phase-high performance liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) to perform qualitative and semi-quantitative analyses of oxylipins in AKO during storage.. A total of 27 oxylipins were identified.

    Article Title: Oxidized linoleic acid metabolites regulate neuronal morphogenesis in vitro
    Article Snippet: 13-HODE (Cayman Chemical, Ann Arbor, MI, USA; Cat #38600); 9-HODE (Cayman Chemical; Cat #38400); 9,10-DiHOME (Cayman Chemical; Cat #53400); 12(13)-EpOME (Cayman Chemical; Cat #52450); 9,10,13-TriHOME (Cayman Chemical; Cat # 26768); 9-OxoODE (Cayman Chemical; Cat #38420); and 12,13-DiHOME (Cayman Chemical; Cat #10009832) were dissolved as 1 mM stocks in absolute ethanol.

    Article Title: Holding glycolysis in check though Alox15 activity is required for macrophage M2 commitment and function in tissue repair and anti-helminth immunity
    Article Snippet: BEC 1 uM (Sigma), Rosiglitazone 0.1, 0.5, 1uM (PPAR-γ agonist, Sigma), GW501516 0.1, 1, 5uM (PPAR-δ agonist, Sigma), n-3 Docosapetaenoic acid sodium salt DPA 100uM (Cayman Chemical) 15-HETE (Cayman Chemical), 13-HODE (Cayman Chemical), 9-HODE (Cayman Chemical) were reconstituted in DMSO (Sigma).

    Article Title: Influence of Lipoxygenase Inhibition on Glioblastoma Cell Biology
    Article Snippet: After 24 h, cells were treated with 0.1 μM, 0.5 μM, and 1 μM of the following (S)-form oxylipins: 13-HODE, 9-HODE, 15-HETE, 5-HETE, 12-HETE, 8-HETE, 9-HETE, 20-HETE (all Cayman Chemical, USA) and 100% ethanol (control).

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: Treatments LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Cell Culture:

    Article Title: Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE.
    Article Snippet: Different fatty acids have distinct effects on the survival of breast cancer cells, which could be mediated by fatty acid binding proteins (FABPs), a family of lipid chaperones.. Due to the diverse structures of the members of FABP family, each FABP demonstrates distinct binding affinities to different fatty acids.. Of note, FABP7 is predominantly expressed in triple negative breast cancer (TNBC), the most aggressive subtype of breast cancer.

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Article Title: Characterization of oxylipins in Antarctic krill oil (Euphausia superba) during storage based on RPLC-MS/MS analysis.
    Article Snippet: Antarctic krill oil (AKO) is rich in polyunsaturated fatty acids (PUFAs), but is prone to oxidative degradation, resulting in the formation of oxylipins, which compromise AKO quality.. Herein, we used reversed-phase-high performance liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) to perform qualitative and semi-quantitative analyses of oxylipins in AKO during storage.. A total of 27 oxylipins were identified.

    Article Title: Oxidized linoleic acid metabolites regulate neuronal morphogenesis in vitro
    Article Snippet: 13-HODE (Cayman Chemical, Ann Arbor, MI, USA; Cat #38600); 9-HODE (Cayman Chemical; Cat #38400); 9,10-DiHOME (Cayman Chemical; Cat #53400); 12(13)-EpOME (Cayman Chemical; Cat #52450); 9,10,13-TriHOME (Cayman Chemical; Cat # 26768); 9-OxoODE (Cayman Chemical; Cat #38420); and 12,13-DiHOME (Cayman Chemical; Cat #10009832) were dissolved as 1 mM stocks in absolute ethanol.

    Article Title: Holding glycolysis in check though Alox15 activity is required for macrophage M2 commitment and function in tissue repair and anti-helminth immunity
    Article Snippet: BEC 1 uM (Sigma), Rosiglitazone 0.1, 0.5, 1uM (PPAR-γ agonist, Sigma), GW501516 0.1, 1, 5uM (PPAR-δ agonist, Sigma), n-3 Docosapetaenoic acid sodium salt DPA 100uM (Cayman Chemical) 15-HETE (Cayman Chemical), 13-HODE (Cayman Chemical), 9-HODE (Cayman Chemical) were reconstituted in DMSO (Sigma).

    Article Title: Influence of Lipoxygenase Inhibition on Glioblastoma Cell Biology
    Article Snippet: After 24 h, cells were treated with 0.1 μM, 0.5 μM, and 1 μM of the following (S)-form oxylipins: 13-HODE, 9-HODE, 15-HETE, 5-HETE, 12-HETE, 8-HETE, 9-HETE, 20-HETE (all Cayman Chemical, USA) and 100% ethanol (control).

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: Treatments LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Chromatography:

    Article Title: Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE.
    Article Snippet: Different fatty acids have distinct effects on the survival of breast cancer cells, which could be mediated by fatty acid binding proteins (FABPs), a family of lipid chaperones.. Due to the diverse structures of the members of FABP family, each FABP demonstrates distinct binding affinities to different fatty acids.. Of note, FABP7 is predominantly expressed in triple negative breast cancer (TNBC), the most aggressive subtype of breast cancer.

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Article Title: Characterization of oxylipins in Antarctic krill oil (Euphausia superba) during storage based on RPLC-MS/MS analysis.
    Article Snippet: Antarctic krill oil (AKO) is rich in polyunsaturated fatty acids (PUFAs), but is prone to oxidative degradation, resulting in the formation of oxylipins, which compromise AKO quality.. Herein, we used reversed-phase-high performance liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) to perform qualitative and semi-quantitative analyses of oxylipins in AKO during storage.. A total of 27 oxylipins were identified.

    Article Title: Oxidized linoleic acid metabolites regulate neuronal morphogenesis in vitro
    Article Snippet: 13-HODE (Cayman Chemical, Ann Arbor, MI, USA; Cat #38600); 9-HODE (Cayman Chemical; Cat #38400); 9,10-DiHOME (Cayman Chemical; Cat #53400); 12(13)-EpOME (Cayman Chemical; Cat #52450); 9,10,13-TriHOME (Cayman Chemical; Cat # 26768); 9-OxoODE (Cayman Chemical; Cat #38420); and 12,13-DiHOME (Cayman Chemical; Cat #10009832) were dissolved as 1 mM stocks in absolute ethanol.

    Article Title: Holding glycolysis in check though Alox15 activity is required for macrophage M2 commitment and function in tissue repair and anti-helminth immunity
    Article Snippet: BEC 1 uM (Sigma), Rosiglitazone 0.1, 0.5, 1uM (PPAR-γ agonist, Sigma), GW501516 0.1, 1, 5uM (PPAR-δ agonist, Sigma), n-3 Docosapetaenoic acid sodium salt DPA 100uM (Cayman Chemical) 15-HETE (Cayman Chemical), 13-HODE (Cayman Chemical), 9-HODE (Cayman Chemical) were reconstituted in DMSO (Sigma).

    Article Title: Influence of Lipoxygenase Inhibition on Glioblastoma Cell Biology
    Article Snippet: After 24 h, cells were treated with 0.1 μM, 0.5 μM, and 1 μM of the following (S)-form oxylipins: 13-HODE, 9-HODE, 15-HETE, 5-HETE, 12-HETE, 8-HETE, 9-HETE, 20-HETE (all Cayman Chemical, USA) and 100% ethanol (control).

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: Treatments LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Mass Spectrometry:

    Article Title: Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE.
    Article Snippet: Different fatty acids have distinct effects on the survival of breast cancer cells, which could be mediated by fatty acid binding proteins (FABPs), a family of lipid chaperones.. Due to the diverse structures of the members of FABP family, each FABP demonstrates distinct binding affinities to different fatty acids.. Of note, FABP7 is predominantly expressed in triple negative breast cancer (TNBC), the most aggressive subtype of breast cancer.

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Article Title: Characterization of oxylipins in Antarctic krill oil (Euphausia superba) during storage based on RPLC-MS/MS analysis.
    Article Snippet: Antarctic krill oil (AKO) is rich in polyunsaturated fatty acids (PUFAs), but is prone to oxidative degradation, resulting in the formation of oxylipins, which compromise AKO quality.. Herein, we used reversed-phase-high performance liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) to perform qualitative and semi-quantitative analyses of oxylipins in AKO during storage.. A total of 27 oxylipins were identified.

    Article Title: Oxidized linoleic acid metabolites regulate neuronal morphogenesis in vitro
    Article Snippet: 13-HODE (Cayman Chemical, Ann Arbor, MI, USA; Cat #38600); 9-HODE (Cayman Chemical; Cat #38400); 9,10-DiHOME (Cayman Chemical; Cat #53400); 12(13)-EpOME (Cayman Chemical; Cat #52450); 9,10,13-TriHOME (Cayman Chemical; Cat # 26768); 9-OxoODE (Cayman Chemical; Cat #38420); and 12,13-DiHOME (Cayman Chemical; Cat #10009832) were dissolved as 1 mM stocks in absolute ethanol.

    Article Title: Holding glycolysis in check though Alox15 activity is required for macrophage M2 commitment and function in tissue repair and anti-helminth immunity
    Article Snippet: BEC 1 uM (Sigma), Rosiglitazone 0.1, 0.5, 1uM (PPAR-γ agonist, Sigma), GW501516 0.1, 1, 5uM (PPAR-δ agonist, Sigma), n-3 Docosapetaenoic acid sodium salt DPA 100uM (Cayman Chemical) 15-HETE (Cayman Chemical), 13-HODE (Cayman Chemical), 9-HODE (Cayman Chemical) were reconstituted in DMSO (Sigma).

    Article Title: Influence of Lipoxygenase Inhibition on Glioblastoma Cell Biology
    Article Snippet: After 24 h, cells were treated with 0.1 μM, 0.5 μM, and 1 μM of the following (S)-form oxylipins: 13-HODE, 9-HODE, 15-HETE, 5-HETE, 12-HETE, 8-HETE, 9-HETE, 20-HETE (all Cayman Chemical, USA) and 100% ethanol (control).

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: Treatments LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Cell Counting:

    Article Title: Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE.
    Article Snippet: Different fatty acids have distinct effects on the survival of breast cancer cells, which could be mediated by fatty acid binding proteins (FABPs), a family of lipid chaperones.. Due to the diverse structures of the members of FABP family, each FABP demonstrates distinct binding affinities to different fatty acids.. Of note, FABP7 is predominantly expressed in triple negative breast cancer (TNBC), the most aggressive subtype of breast cancer.

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Article Title: Characterization of oxylipins in Antarctic krill oil (Euphausia superba) during storage based on RPLC-MS/MS analysis.
    Article Snippet: Antarctic krill oil (AKO) is rich in polyunsaturated fatty acids (PUFAs), but is prone to oxidative degradation, resulting in the formation of oxylipins, which compromise AKO quality.. Herein, we used reversed-phase-high performance liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) to perform qualitative and semi-quantitative analyses of oxylipins in AKO during storage.. A total of 27 oxylipins were identified.

    Article Title: Oxidized linoleic acid metabolites regulate neuronal morphogenesis in vitro
    Article Snippet: 13-HODE (Cayman Chemical, Ann Arbor, MI, USA; Cat #38600); 9-HODE (Cayman Chemical; Cat #38400); 9,10-DiHOME (Cayman Chemical; Cat #53400); 12(13)-EpOME (Cayman Chemical; Cat #52450); 9,10,13-TriHOME (Cayman Chemical; Cat # 26768); 9-OxoODE (Cayman Chemical; Cat #38420); and 12,13-DiHOME (Cayman Chemical; Cat #10009832) were dissolved as 1 mM stocks in absolute ethanol.

    Article Title: Holding glycolysis in check though Alox15 activity is required for macrophage M2 commitment and function in tissue repair and anti-helminth immunity
    Article Snippet: BEC 1 uM (Sigma), Rosiglitazone 0.1, 0.5, 1uM (PPAR-γ agonist, Sigma), GW501516 0.1, 1, 5uM (PPAR-δ agonist, Sigma), n-3 Docosapetaenoic acid sodium salt DPA 100uM (Cayman Chemical) 15-HETE (Cayman Chemical), 13-HODE (Cayman Chemical), 9-HODE (Cayman Chemical) were reconstituted in DMSO (Sigma).

    Article Title: Influence of Lipoxygenase Inhibition on Glioblastoma Cell Biology
    Article Snippet: After 24 h, cells were treated with 0.1 μM, 0.5 μM, and 1 μM of the following (S)-form oxylipins: 13-HODE, 9-HODE, 15-HETE, 5-HETE, 12-HETE, 8-HETE, 9-HETE, 20-HETE (all Cayman Chemical, USA) and 100% ethanol (control).

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: Treatments LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Migration:

    Article Title: Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE.
    Article Snippet: Different fatty acids have distinct effects on the survival of breast cancer cells, which could be mediated by fatty acid binding proteins (FABPs), a family of lipid chaperones.. Due to the diverse structures of the members of FABP family, each FABP demonstrates distinct binding affinities to different fatty acids.. Of note, FABP7 is predominantly expressed in triple negative breast cancer (TNBC), the most aggressive subtype of breast cancer.

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Article Title: Characterization of oxylipins in Antarctic krill oil (Euphausia superba) during storage based on RPLC-MS/MS analysis.
    Article Snippet: Antarctic krill oil (AKO) is rich in polyunsaturated fatty acids (PUFAs), but is prone to oxidative degradation, resulting in the formation of oxylipins, which compromise AKO quality.. Herein, we used reversed-phase-high performance liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) to perform qualitative and semi-quantitative analyses of oxylipins in AKO during storage.. A total of 27 oxylipins were identified.

    Article Title: Oxidized linoleic acid metabolites regulate neuronal morphogenesis in vitro
    Article Snippet: 13-HODE (Cayman Chemical, Ann Arbor, MI, USA; Cat #38600); 9-HODE (Cayman Chemical; Cat #38400); 9,10-DiHOME (Cayman Chemical; Cat #53400); 12(13)-EpOME (Cayman Chemical; Cat #52450); 9,10,13-TriHOME (Cayman Chemical; Cat # 26768); 9-OxoODE (Cayman Chemical; Cat #38420); and 12,13-DiHOME (Cayman Chemical; Cat #10009832) were dissolved as 1 mM stocks in absolute ethanol.

    Article Title: Holding glycolysis in check though Alox15 activity is required for macrophage M2 commitment and function in tissue repair and anti-helminth immunity
    Article Snippet: BEC 1 uM (Sigma), Rosiglitazone 0.1, 0.5, 1uM (PPAR-γ agonist, Sigma), GW501516 0.1, 1, 5uM (PPAR-δ agonist, Sigma), n-3 Docosapetaenoic acid sodium salt DPA 100uM (Cayman Chemical) 15-HETE (Cayman Chemical), 13-HODE (Cayman Chemical), 9-HODE (Cayman Chemical) were reconstituted in DMSO (Sigma).

    Article Title: Influence of Lipoxygenase Inhibition on Glioblastoma Cell Biology
    Article Snippet: After 24 h, cells were treated with 0.1 μM, 0.5 μM, and 1 μM of the following (S)-form oxylipins: 13-HODE, 9-HODE, 15-HETE, 5-HETE, 12-HETE, 8-HETE, 9-HETE, 20-HETE (all Cayman Chemical, USA) and 100% ethanol (control).

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: Treatments LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Expressing:

    Article Title: Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE.
    Article Snippet: Different fatty acids have distinct effects on the survival of breast cancer cells, which could be mediated by fatty acid binding proteins (FABPs), a family of lipid chaperones.. Due to the diverse structures of the members of FABP family, each FABP demonstrates distinct binding affinities to different fatty acids.. Of note, FABP7 is predominantly expressed in triple negative breast cancer (TNBC), the most aggressive subtype of breast cancer.

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Article Title: Characterization of oxylipins in Antarctic krill oil (Euphausia superba) during storage based on RPLC-MS/MS analysis.
    Article Snippet: Antarctic krill oil (AKO) is rich in polyunsaturated fatty acids (PUFAs), but is prone to oxidative degradation, resulting in the formation of oxylipins, which compromise AKO quality.. Herein, we used reversed-phase-high performance liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) to perform qualitative and semi-quantitative analyses of oxylipins in AKO during storage.. A total of 27 oxylipins were identified.

    Article Title: Oxidized linoleic acid metabolites regulate neuronal morphogenesis in vitro
    Article Snippet: 13-HODE (Cayman Chemical, Ann Arbor, MI, USA; Cat #38600); 9-HODE (Cayman Chemical; Cat #38400); 9,10-DiHOME (Cayman Chemical; Cat #53400); 12(13)-EpOME (Cayman Chemical; Cat #52450); 9,10,13-TriHOME (Cayman Chemical; Cat # 26768); 9-OxoODE (Cayman Chemical; Cat #38420); and 12,13-DiHOME (Cayman Chemical; Cat #10009832) were dissolved as 1 mM stocks in absolute ethanol.

    Article Title: Holding glycolysis in check though Alox15 activity is required for macrophage M2 commitment and function in tissue repair and anti-helminth immunity
    Article Snippet: BEC 1 uM (Sigma), Rosiglitazone 0.1, 0.5, 1uM (PPAR-γ agonist, Sigma), GW501516 0.1, 1, 5uM (PPAR-δ agonist, Sigma), n-3 Docosapetaenoic acid sodium salt DPA 100uM (Cayman Chemical) 15-HETE (Cayman Chemical), 13-HODE (Cayman Chemical), 9-HODE (Cayman Chemical) were reconstituted in DMSO (Sigma).

    Article Title: Influence of Lipoxygenase Inhibition on Glioblastoma Cell Biology
    Article Snippet: After 24 h, cells were treated with 0.1 μM, 0.5 μM, and 1 μM of the following (S)-form oxylipins: 13-HODE, 9-HODE, 15-HETE, 5-HETE, 12-HETE, 8-HETE, 9-HETE, 20-HETE (all Cayman Chemical, USA) and 100% ethanol (control).

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: Treatments LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Staining:

    Article Title: Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE.
    Article Snippet: Different fatty acids have distinct effects on the survival of breast cancer cells, which could be mediated by fatty acid binding proteins (FABPs), a family of lipid chaperones.. Due to the diverse structures of the members of FABP family, each FABP demonstrates distinct binding affinities to different fatty acids.. Of note, FABP7 is predominantly expressed in triple negative breast cancer (TNBC), the most aggressive subtype of breast cancer.

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Article Title: Characterization of oxylipins in Antarctic krill oil (Euphausia superba) during storage based on RPLC-MS/MS analysis.
    Article Snippet: Antarctic krill oil (AKO) is rich in polyunsaturated fatty acids (PUFAs), but is prone to oxidative degradation, resulting in the formation of oxylipins, which compromise AKO quality.. Herein, we used reversed-phase-high performance liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) to perform qualitative and semi-quantitative analyses of oxylipins in AKO during storage.. A total of 27 oxylipins were identified.

    Article Title: Oxidized linoleic acid metabolites regulate neuronal morphogenesis in vitro
    Article Snippet: 13-HODE (Cayman Chemical, Ann Arbor, MI, USA; Cat #38600); 9-HODE (Cayman Chemical; Cat #38400); 9,10-DiHOME (Cayman Chemical; Cat #53400); 12(13)-EpOME (Cayman Chemical; Cat #52450); 9,10,13-TriHOME (Cayman Chemical; Cat # 26768); 9-OxoODE (Cayman Chemical; Cat #38420); and 12,13-DiHOME (Cayman Chemical; Cat #10009832) were dissolved as 1 mM stocks in absolute ethanol.

    Article Title: Holding glycolysis in check though Alox15 activity is required for macrophage M2 commitment and function in tissue repair and anti-helminth immunity
    Article Snippet: BEC 1 uM (Sigma), Rosiglitazone 0.1, 0.5, 1uM (PPAR-γ agonist, Sigma), GW501516 0.1, 1, 5uM (PPAR-δ agonist, Sigma), n-3 Docosapetaenoic acid sodium salt DPA 100uM (Cayman Chemical) 15-HETE (Cayman Chemical), 13-HODE (Cayman Chemical), 9-HODE (Cayman Chemical) were reconstituted in DMSO (Sigma).

    Article Title: Influence of Lipoxygenase Inhibition on Glioblastoma Cell Biology
    Article Snippet: After 24 h, cells were treated with 0.1 μM, 0.5 μM, and 1 μM of the following (S)-form oxylipins: 13-HODE, 9-HODE, 15-HETE, 5-HETE, 12-HETE, 8-HETE, 9-HETE, 20-HETE (all Cayman Chemical, USA) and 100% ethanol (control).

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: Treatments LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Light Microscopy:

    Article Title: Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE.
    Article Snippet: Different fatty acids have distinct effects on the survival of breast cancer cells, which could be mediated by fatty acid binding proteins (FABPs), a family of lipid chaperones.. Due to the diverse structures of the members of FABP family, each FABP demonstrates distinct binding affinities to different fatty acids.. Of note, FABP7 is predominantly expressed in triple negative breast cancer (TNBC), the most aggressive subtype of breast cancer.

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Article Title: Characterization of oxylipins in Antarctic krill oil (Euphausia superba) during storage based on RPLC-MS/MS analysis.
    Article Snippet: Antarctic krill oil (AKO) is rich in polyunsaturated fatty acids (PUFAs), but is prone to oxidative degradation, resulting in the formation of oxylipins, which compromise AKO quality.. Herein, we used reversed-phase-high performance liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) to perform qualitative and semi-quantitative analyses of oxylipins in AKO during storage.. A total of 27 oxylipins were identified.

    Article Title: Oxidized linoleic acid metabolites regulate neuronal morphogenesis in vitro
    Article Snippet: 13-HODE (Cayman Chemical, Ann Arbor, MI, USA; Cat #38600); 9-HODE (Cayman Chemical; Cat #38400); 9,10-DiHOME (Cayman Chemical; Cat #53400); 12(13)-EpOME (Cayman Chemical; Cat #52450); 9,10,13-TriHOME (Cayman Chemical; Cat # 26768); 9-OxoODE (Cayman Chemical; Cat #38420); and 12,13-DiHOME (Cayman Chemical; Cat #10009832) were dissolved as 1 mM stocks in absolute ethanol.

    Article Title: Holding glycolysis in check though Alox15 activity is required for macrophage M2 commitment and function in tissue repair and anti-helminth immunity
    Article Snippet: BEC 1 uM (Sigma), Rosiglitazone 0.1, 0.5, 1uM (PPAR-γ agonist, Sigma), GW501516 0.1, 1, 5uM (PPAR-δ agonist, Sigma), n-3 Docosapetaenoic acid sodium salt DPA 100uM (Cayman Chemical) 15-HETE (Cayman Chemical), 13-HODE (Cayman Chemical), 9-HODE (Cayman Chemical) were reconstituted in DMSO (Sigma).

    Article Title: Influence of Lipoxygenase Inhibition on Glioblastoma Cell Biology
    Article Snippet: After 24 h, cells were treated with 0.1 μM, 0.5 μM, and 1 μM of the following (S)-form oxylipins: 13-HODE, 9-HODE, 15-HETE, 5-HETE, 12-HETE, 8-HETE, 9-HETE, 20-HETE (all Cayman Chemical, USA) and 100% ethanol (control).

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: Treatments LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Wound Healing Assay:

    Article Title: Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE.
    Article Snippet: Different fatty acids have distinct effects on the survival of breast cancer cells, which could be mediated by fatty acid binding proteins (FABPs), a family of lipid chaperones.. Due to the diverse structures of the members of FABP family, each FABP demonstrates distinct binding affinities to different fatty acids.. Of note, FABP7 is predominantly expressed in triple negative breast cancer (TNBC), the most aggressive subtype of breast cancer.

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.

    Article Title: Characterization of oxylipins in Antarctic krill oil (Euphausia superba) during storage based on RPLC-MS/MS analysis.
    Article Snippet: Antarctic krill oil (AKO) is rich in polyunsaturated fatty acids (PUFAs), but is prone to oxidative degradation, resulting in the formation of oxylipins, which compromise AKO quality.. Herein, we used reversed-phase-high performance liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) to perform qualitative and semi-quantitative analyses of oxylipins in AKO during storage.. A total of 27 oxylipins were identified.

    Article Title: Oxidized linoleic acid metabolites regulate neuronal morphogenesis in vitro
    Article Snippet: 13-HODE (Cayman Chemical, Ann Arbor, MI, USA; Cat #38600); 9-HODE (Cayman Chemical; Cat #38400); 9,10-DiHOME (Cayman Chemical; Cat #53400); 12(13)-EpOME (Cayman Chemical; Cat #52450); 9,10,13-TriHOME (Cayman Chemical; Cat # 26768); 9-OxoODE (Cayman Chemical; Cat #38420); and 12,13-DiHOME (Cayman Chemical; Cat #10009832) were dissolved as 1 mM stocks in absolute ethanol.

    Article Title: Holding glycolysis in check though Alox15 activity is required for macrophage M2 commitment and function in tissue repair and anti-helminth immunity
    Article Snippet: BEC 1 uM (Sigma), Rosiglitazone 0.1, 0.5, 1uM (PPAR-γ agonist, Sigma), GW501516 0.1, 1, 5uM (PPAR-δ agonist, Sigma), n-3 Docosapetaenoic acid sodium salt DPA 100uM (Cayman Chemical) 15-HETE (Cayman Chemical), 13-HODE (Cayman Chemical), 9-HODE (Cayman Chemical) were reconstituted in DMSO (Sigma).

    Article Title: Influence of Lipoxygenase Inhibition on Glioblastoma Cell Biology
    Article Snippet: After 24 h, cells were treated with 0.1 μM, 0.5 μM, and 1 μM of the following (S)-form oxylipins: 13-HODE, 9-HODE, 15-HETE, 5-HETE, 12-HETE, 8-HETE, 9-HETE, 20-HETE (all Cayman Chemical, USA) and 100% ethanol (control).

    Article Title: Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner
    Article Snippet: Treatments LA (NuChek, Elysian, MN, USA), 13-HODE (Cayman Chemical, Ann Arbor, MI, USA), or PGE2 (Cayman Chemical) were first dissolved as 1000X stocks in absolute ethanol and then diluted at 1:1000 directly into cultures.



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    Image Search Results


    PPARG-mediated lipid metabolism reprograms CD36⁺ CAFs through the CD36–FABP4 axis. a – d PPARG activity and expression in CAFs, showing positive correlations with CD36 and FABP4. e – f qRT-PCR validation of PPARG, CD36, and FABP4 expression in ( e ) CD36⁺ CAFs ( n = 5 per group, ** p < 0.01) and ( f ) in response to OxLDL treatment (50 µg/mL; n = 3 per group, ** p < 0.01, * p < 0.05). g Western blot analysis of CD36, PPARG, ANGPTL4, and FABP4 expression in CAFs treated with OxLDL, siCD36, or CD36 inhibitor (SSO) ( n = 3 per group). h – i qRT-PCR and Western blot showing that PPARG knockdown reduces CD36, FABP4, and ANGPTL4 expression ( n = 5 per group, **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05). j ChIP-qPCR confirming PPARG binding to CD36 and FABP4 promoters ( n = 3 per group, ** p < 0.01, * p < 0.05). k ELISA showing increased 13-HODE levels in CAFs after OxLDL stimulation ( n = 3 per group, *** p < 0.001). (l–m) qRT-PCR and Western blot showing that 13-HODE induces CD36, FABP4, and ANGPTL4 expression ( n = 5 per group, **** p < 0.0001, *** p < 0.001). n – o qRT-PCR and Western blot showing that FABP4 knockdown impairs 13-HODE–induced CD36 and ANGPTL4 expression ( n = 5 per group, **** p < 0.0001, *** p < 0.001). p Molecular docking analysis predicting binding interactions among 13-HODE, FABP4, and the PPARG ligand-binding domain (LBD). q – r AlphaFold modeling, and co-IP validating FABP4–PPARG interaction. See also Supplementary Figure S5

    Journal: Molecular Cancer

    Article Title: Targeting OxLDL-mediated CD36 + CAF reprogramming potentiates PD-1 immunotherapy in osteosarcoma

    doi: 10.1186/s12943-025-02516-2

    Figure Lengend Snippet: PPARG-mediated lipid metabolism reprograms CD36⁺ CAFs through the CD36–FABP4 axis. a – d PPARG activity and expression in CAFs, showing positive correlations with CD36 and FABP4. e – f qRT-PCR validation of PPARG, CD36, and FABP4 expression in ( e ) CD36⁺ CAFs ( n = 5 per group, ** p < 0.01) and ( f ) in response to OxLDL treatment (50 µg/mL; n = 3 per group, ** p < 0.01, * p < 0.05). g Western blot analysis of CD36, PPARG, ANGPTL4, and FABP4 expression in CAFs treated with OxLDL, siCD36, or CD36 inhibitor (SSO) ( n = 3 per group). h – i qRT-PCR and Western blot showing that PPARG knockdown reduces CD36, FABP4, and ANGPTL4 expression ( n = 5 per group, **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05). j ChIP-qPCR confirming PPARG binding to CD36 and FABP4 promoters ( n = 3 per group, ** p < 0.01, * p < 0.05). k ELISA showing increased 13-HODE levels in CAFs after OxLDL stimulation ( n = 3 per group, *** p < 0.001). (l–m) qRT-PCR and Western blot showing that 13-HODE induces CD36, FABP4, and ANGPTL4 expression ( n = 5 per group, **** p < 0.0001, *** p < 0.001). n – o qRT-PCR and Western blot showing that FABP4 knockdown impairs 13-HODE–induced CD36 and ANGPTL4 expression ( n = 5 per group, **** p < 0.0001, *** p < 0.001). p Molecular docking analysis predicting binding interactions among 13-HODE, FABP4, and the PPARG ligand-binding domain (LBD). q – r AlphaFold modeling, and co-IP validating FABP4–PPARG interaction. See also Supplementary Figure S5

    Article Snippet: Blood samples were collected from cardiac puncture separated by centrifugation at 3000 g, 4°C, 10 min. OxLDL (Elabscience, E-EL-M0066), 13-HODE (MEIMIAN, MM-60095H2) and ANGPTL4 (Proteintech, KE00186) levels were measured using ELISA kits following the manufacturer’s instructions.

    Techniques: Activity Assay, Expressing, Quantitative RT-PCR, Biomarker Discovery, Western Blot, Knockdown, ChIP-qPCR, Binding Assay, Enzyme-linked Immunosorbent Assay, Ligand Binding Assay, Co-Immunoprecipitation Assay